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You are here:Home » Antibodies » Abs to Enzymes, Angiotensin Converting (ACE) » Anti -Angiotensin Converting Enzyme (ACE, CD143) (PE)

Anti -Angiotensin Converting Enzyme (ACE, CD143) (PE)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified FC
Angiotensin I Converting Enzyme (ACE, also known as peptidyl-dipeptidase A, kininase II, or CD143) is a type I transmembrane zinc metallopeptidase that is important in the metabolism of the vaso-active peptides angiotensin II and bradykinin (1, 2). Two isoforms of ACE that exhibit distinct physiologic functions have been described: germinally expressed ACE (gACE) and somatic cell expressed ACE (sACE) (2,3). Both isoforms are transcribed from a single gene using alternative promoters (2, 3). The gACE isoform is exclusively expressed in the testes, whereas sACE is expressed on endothelial cells, epithelial cells, neuronal cells, and dendritic cells (4, 5). A soluble form of CD143 is likely shed from endothelial cells and is found in serum, plasma, seminal fluid, amniotic fluid and cerebral spinal fluid (1). Functionally, CD143 has a role in blood
pressure regulation, electrolyte balance, reproduction, cardiovascular disease, kidney disease, cancer and Alzheimer’s disease (6-10). Inhibitors of ACE are used clinically to treat hypertension, cardiac failure, and diabetic retinopathy (6, 11).
Catalog #A2295-01D3
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: Designed to quantitatively determine the percentage of cells bearing CD143 within a population and qualitatively determine the density of CD143 on cell surfaces by flow
cytometry. Washed cells are incubated with the phycoerythrin-labeled monoclonal antibody, which binds to cells expressing CD143. Unbound phycoerythrin-conjugated antibody is then washed from the cells. Cells expressing CD143 are fluorescently stained, with the intensity of
staining directly proportional to the density of expression of CD143. Cell surface expression of CD143 is determined by flow cytometric analysis using 488nm wavelength laser excitation.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9H61
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in 1.0ml of PE-labeled antibody at a concentration of 25ug/ml.
PurityPurified
ImmunogenSynthetic peptide corresponding to human ACE/CD143.
SpecificityRecognizes human ACE/CD143.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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