| Human CD143, also known as angiotensin-converting enzyme (ACE). The somatic form is expressed by endothelial cells (especially those of arterioles and lung capillaries), epithelial cells (especially in proximal renal tubules and in the small intestine), by some neuronal cells and variably on some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa. Study of CD143 may be relevant to fertility (especially sperm-egg interactions), atherosclerosis and in drug targeting to the lung. This clone has been reported to recognize ACE in Western Blot. |
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| Catalog # | A2295-01T |
| Applications | Suitable for use in Immunohistochemistry, Western Blot and |
| ELISA. Other applications not tested. |
| Recommended Dilutions | Immunohistochemistry (Paraffin): 1:10-1:100. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended. |
| Western Blot: 1:50-1:100 |
| Optimal working dilutions to be determined by researcher. |
| Recommended Positive Control Tissue | Human Kidney |
| Recommended Negative Controls | I1904-79G: Mouse IgG1 Negative Control |
| Recommended Secondary Reagents | F(ab')2 rabbit anti-mouse IgG HRP |
| Hybridoma | |
| Spleen cells from immunized Balb/c mice were fused with cells of the mouse X36-Ag8-653 myeloma cell line. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H533 |
| Host | Mouse |
| Source | Human |
| Concentration | Not Determined |
| Form | Supplied as a liquid, 0.1% sodium azide. |
| Purity | Tissue culture supernatant. |
| Immunogen | Denatured human kidney CD143 (Angiotensin converting enzyme). |
| Specificity | Recognizes human CD143, also known as angiotensin-converting enzyme (ACE). CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The antibody recognizes denatured ACE, binding to an epitope in the C domain. |
| Species Crossreactivity | Monkey, rabbit, bovine, canine, feline, guinea pig, goat and sheep. Does not react with porcine and rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
