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You are here:Home » Antibodies » Abs to Enzymes, Angiotensin Converting (ACE) » Anti -Angiotensin Converting Enzyme (ACE, CD143)

Anti -Angiotensin Converting Enzyme (ACE, CD143)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant E B IH
Human CD143, also known as angiotensin-converting enzyme (ACE). The somatic form is expressed by endothelial cells (especially those of arterioles and lung capillaries), epithelial cells (especially in proximal renal tubules and in the small intestine), by some neuronal cells and variably on some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa. Study of CD143 may be relevant to fertility (especially sperm-egg interactions), atherosclerosis and in drug targeting to the lung. This clone has been reported to recognize ACE in Western Blot.
Catalog #A2295-01T
ApplicationsSuitable for use in Immunohistochemistry, Western Blot and
ELISA. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Paraffin): 1:10-1:100. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended.
Western Blot: 1:50-1:100
Optimal working dilutions to be determined by researcher.
Recommended Positive Control TissueHuman Kidney
Recommended Negative ControlsI1904-79G: Mouse IgG1 Negative Control
Recommended Secondary ReagentsF(ab')2 rabbit anti-mouse IgG HRP
Hybridoma
Spleen cells from immunized Balb/c mice were fused with cells of the mouse X36-Ag8-653 myeloma cell line.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H533
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid, 0.1% sodium azide.
PurityTissue culture supernatant.
ImmunogenDenatured human kidney CD143 (Angiotensin converting enzyme).
SpecificityRecognizes human CD143, also known as angiotensin-converting enzyme (ACE). CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The antibody recognizes denatured ACE, binding to an epitope in the C domain.
Species CrossreactivityMonkey, rabbit, bovine, canine, feline, guinea pig, goat and sheep. Does not react with porcine and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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