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You are here:Home » Antibodies » Abs to Enzymes, Angiotensin Converting (ACE) » Anti -Angiotensin Converting Enzyme 2 (ACE-2, ACEH)

Anti -Angiotensin Converting Enzyme 2 (ACE-2, ACEH)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B FC
ACE-2 (also known as ACE-2 and ACE homolog, ACEH) gene has been mapped at human chromosome Xp22. ACE-2 enzymes from human (805aa) and mouse (798aa) are single chain proteins with 40% sequence homology to N- and C-terminal domains of ACE. However, in contrast to s-ACE which consists of two catalytic sites, ACE-2 contains only one active site. Unlike s-ACE and t-ACE which are dipeptidyl-carboxypeptidases, ACE-2 acts as a carboxypeptidase, cleaving single residue from Ang-I, generating Ang1-9 and a single residue from Ang-II to generate Ang1-7. ACE-2 can cleave angiotensin-I, but not bradykinin and the enzyme activity is not inhibited by the ACE inhibitors. This enzyme is expressed highly in heart, kidney and testis and moderately in colon, small intestine and ovary. ACE-2 is an essential regulator of heart fuction because targeted disruption of this enzyme in mice results in severe cardiac contractility defect, increased angiotensin-II levels and upregulation of hypoxia-induced genes in the heart.
Catalog #A2295-01Y
ApplicationsSuitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA (Direct and Indirect): 1:2000-1:10,000
Western Blot: 1:2000-1:10,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween-20 and 5% skim milk; suggested incubation time is 1 hour at RT. Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6D118
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. No preservatives.
PurityPurified
ImmunogenRecombinant human ACE2 (angiotensin-converting enzyme 2).
SpecificityRecognizes human ACE2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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