| CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expresssed by spermatozoa. Study of CD143 may be relevant to fertility (especially sperm-egg interactions), atherosclerosis and in drug targeting to the lung. |
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| Catalog # | A2295-02C |
| Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:50-1:100 |
| Immunohistochemistry (Paraffin): Neat. 1:20. Requites antigen retrieval using heat treatment methods prior to staining. |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | Spleen cells from immunized Balb/c mice were fused with cells of the mouse X63-Ag8-653 myeloma cell line. |
| Recommended Positive Control Tissue | Kidney (lung in rat studies) |
| Recommended Negative Control | I1904-79G: IgG1, Mouse, Negative Control |
| Recommended Secondary Reagents | I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo |
| I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H536 |
| Host | Mouse |
| Source | Human |
| Concentration | Not Determined |
| Form | Supplied as a liquid with 0.1% sodium azide. |
| Purity | Tissue culture supernatant. |
| Immunogen | Denatured Human kidney CD143 (angiotensin converting enzyme). |
| Specificity | Recognizes human CD143, also know as angiotensin-converting enzyme (ACE). This clone recognizes denatured ACE, binding to an epitope in the C domain and has been reported to be active in Western Blot. Species Crossreactivity: monkey, rabbit , bovine, canine, feline, guinea pig, rat, porcine and mouse |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
