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You are here:Home » Antibodies » Abs to Enzymes, Angiotensin Converting (ACE) » Anti -ACE2, NT (Angiotensin-converting Enzyme 2, ACE-related Carboxypeptidase, Angiotensin-converting Enzyme Homolog, ACEH, Metalloprotease MPROT15, Processed Angiotensin-converting Enzyme 2, UNQ868/PRO1885) (Azide free) (HRP)

Anti -ACE2, NT (Angiotensin-converting Enzyme 2, ACE-related Carboxypeptidase,
Angiotensin-converting Enzyme Homolog, ACEH, Metalloprotease MPROT15, Processed
Angiotensin-converting Enzyme 2, UNQ868/PRO1885) (Azide free) (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
ACE2 cDNA encodes a deduced 805aa protein containing a potential 17aa N-terminal signal peptide and a putative 22aa C-terminal membrane anchor. It also possesses a zinc metalloprotease consensus sequence and a conserved glutamine residue that may function as a third zinc ligand. ACE2 is expressed predominantly in vascular endothelial cells of the heart and kidney. ACE converts angiotensin I to angiotensin II, ACE2 converts angiotensin I to angiotensin 1-9, which has 9aa. Angiotensin II is a potent blood vessel constrictor, while angiotensin 1-9 does not impact blood vessels but is cleaved by ACE to a shorter peptide, angiotensin 1-7, which is a blood vessel dilator. Spike (S) proteins of coronaviruses, including the SARS coronavirus, bind with cellular receptors to mediate infection of target cells. ACE2 binds the S1 domain of the SARS coronavirus S protein. SARS coronavirus replicates efficiently on ACE2-transfected but not mock-transfected 293T cells. Anti-ACE2 but not anti-ACE1 antibody blocks viral replication on Vero E6 cells. It has been proposed that ACE2 is a functional receptor for SARS coronavirus.
Catalog #A2295-02D1-HRP
ApplicationsSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:1,000
Western Blot: 1:100-1:250
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). Conjugated to HRP.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide selected from the N-terminal region of human ACE2 (KLH).
SpecificityRecognizes human ACE2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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