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You are here:Home » Antibodies » Abs to Enzymes, Angiotensin Converting (ACE) » Anti -Angiotensin Converting Enzyme (ACE, CD143) (Biotin)

Anti -Angiotensin Converting Enzyme (ACE, CD143) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified FC
CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The somatic form is expressed by endothelial cells (especially those of arterioles and lung capillaries), epithehal cells (especially in proximal renal tubules and in the small intestine), by some neuronal cells and variably on some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.
Catalog #A2295-02E
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: Neat.1:10. Use 10ul to label 10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.
HybridomaSpleen cells from immunized Balb/c mice were fused with cells of the mouse X36-Ag8-653 myeloma cell line.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H534
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid, 0.1% sodium azide, 1% BSA. Labeled with Biotin.
PurityPurified
ImmunogenHuman lung CD143 (Angiotensin converting enzyme).
SpecificityRecognizes human CD143, also known as angiotensin-converting enzyme (ACE). Also recognizes active ACE binding to an N-terminal domain epitope different to that recognized by clone 9B9. Species Crossreactivity: monkey
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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