Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Enzymes, Angiotensin Converting (ACE) » Anti -Angiotensin Converting Enzyme (ACE, CD143) (Biotin)

Anti -Angiotensin Converting Enzyme (ACE, CD143) (Biotin)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expresssed by spermatozoa.
Catalog #A2295-02F
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:10 (neat). Use 10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.
HybridomaP3X63-Ag8.653. Myeloma cells with spleen cells from Balb/c mice.
Recommended Negative ControlI1904-78M: Mouse IgG1 Negative Control
Recommended Secondary ReagentS7973-96B: Streptavidin: FITC
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H539
HostMouse
SourceHuman
Concentration~0.1mg/ml.
FormSupplied as a liquid in 0.09% sodium azide, 1% BSA. Labeled with Biotin.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman lung CD143.
SpecificityRecognizes human CD143 (ACE). Reacts with active ACE by binding to an N-terminal domain epitope, different to that recognized by clone i2H5. Species Crossreactivity: Monkey, rat, hamster, feline.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links