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You are here:Home » Antibodies » Abs to Enzymes, Angiotensin Converting (ACE) » Anti -Angiotensin Converting Enzyme (ACE, CD143) (FITC)

Anti -Angiotensin Converting Enzyme (ACE, CD143) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
Catalog #A2295-02H
Fusion Partners Spleen cells from immunized Balb/c mice were fused with cells of the mouse X63-Ag8-653 myeloma cell line.
Recommended Negative Controls Mouse IgG1 Negative Control
ApplicationsFlow Cytometry: Neat
Optimal working dilutions to be determined by researcher.
Flow Cytometry Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H535
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormPurified IgG conjugated to FluoresceIsothiocyanate Isomer 1 (FITC). Supplied as a liquid, 0.5% BSA, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman lung CD143.
SpecificityRecognizes human CD143, also know as angiotensin-converting enzyme (ACE). CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expresssed by spermatozoa. This antibody recognizes active ACE binding to an N-terminal domain epitope, different to that recognized by clone i2H5. Species Crossreactivity: Human, monkey (3), rat (3), hamster, cat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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