Angiogensin I Converting Enzyme-2 (ACE-2) is a type I transmembrane zinc protease that cleaves angiotensins I and II to produce vasodilatory and anti-proliferative peptides. The balance between ACE-1 and ACE-2 activity is critical for maintaining cardiovascular, renal, and pulmonary function. ACE-2 isoforms of 75kD and 120kD are differentially expressed between lung and kidney, respectively, and a shed soluble form is generated by TACE/ADAM17 mediated cleavage. ACE-2 also functions as the cellular uptake receptor for the SARS coronoavirus. Within the extracellular domain, human ACE-2 shares 83% aa sequence identity with mouse and rat ACE-2.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 2ug/ml in NS0 mouse myeloma cell line and human kidney tissue Immunohistochemistry: 8-25ug/ml in immersion fixed paraffin-embedded sections of human kidney and hamster lung. Optimal dilutions to be determined by the researcher.
Hybridoma
Mouse myeloma with B cells obtained from a mouse.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Gln18-Ser740 of human ACE-2 |expressed in NS0 cells.
Form
Supplied as a lyophilized powder from PBS, pH 6.2, 5% trehalose. Reconstitute with 1ml sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human ACE-2 ectodomain. No cross-reactivity with recombinant human (rh) ACE-1 or rhNeprilysin is observed in Western Blot. Species Crossreactivity: hamster