| Angiogensin I Converting Enzyme-2 (ACE-2) is a type I transmembrane zinc protease that |
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| cleaves angiotensins I and II to produce vasodilatory and anti-proliferative peptides. The |
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| balance between ACE-1 and ACE-2 activity is critical for maintaining cardiovascular, renal, |
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| and pulmonary function. ACE-2 isoforms of 75kD and 120kD are differentially expressed |
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| between lung and kidney, respectively, and a shed soluble form is generated by |
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| TACE/ADAM17 mediated cleavage. ACE-2 also functions as the cellular uptake receptor for |
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| the SARS coronoavirus. Within the extracellular domain, human ACE-2 shares 83% aa |
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| sequence identity with mouse and rat ACE-2. |
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| |
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| Applications: |
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| Suitable for use in ELISA, Western Blot, Immunohistochemistry. Other applications not tested. |
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| Catalog # | A2295-02S1 |
| Recommended Dilution | ELISA: 0.5-1.0ug/ml |
| Western Blot: 2ug/ml |
| Immunohistochemistry: 25ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | Mouse myeloma with B cells obtained from a mouse. |
| Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 9H63 |
| Host | Mouse |
| Source | Human |
| Concentration | ~500ug/ml |
| Form | Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with 40-50% glycerol, PBS. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | NS0-derived recombinant human ACE-2 aa18-740; Q9BYF1. |
| Specificity | Recognizes human ACE-2 ectodomain. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
