Angiotensin Converting Enzyme 2, Ectodomain (ACE2, ACE-2, ACE-related Carboxypeptidase,
Angiotensin Converting Enzyme Homolog, ACEH, DKFZP434A014, EC 3.4.17, OTTHUMP00000022963)
(Biotin)
Pricing
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| Angiogensin I Converting Enzyme-2 (ACE-2) is a type I transmembrane zinc protease that cleaves angiotensins I and II to produce vasodilatory and anti-proliferative peptides. The balance between ACE-1 and ACE-2 activity is critical for maintaining cardiovascular, renal, and pulmonary function. ACE-2 isoforms of 75kD and 120kD are differentially expressed between lung and kidney, respectively, and a shed soluble form is generated by TACE/ADAM17 mediated cleavage. ACE-2 also functions as the cellular uptake receptor for the SARS coronoavirus. Within the extracellular domain, human ACE-2 shares 83% aa sequence identity with mouse and rat ACE-2. | | | Catalog # | A2295-02S3 | | Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 0.1-0.2ug/ml. The detection limit is ~1ng/lane under non-reducing and reducing conditions. Shows <1% crossreactivity with recombinant human ACE. | | Immunohistochemistry: 5-15ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS, pH 7.3, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Product Type | Pab | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | ~0.05mg/ml | | Form | Supplied as a lyophilized powder from PBS, 2.5mg BSA. Labeled with Biotin. Reconstitute with 1ml sterile TBS, pH 7.3, 0.1% BSA. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant corresponding to aa18-740 of human ACE-2 ectodomain expressed in NSO cells. | | Specificity | Recognizes human ACE-2 ectodomain. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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