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You are here:Home » Molecular Biology » MB-Enzymes, Angiotensin Converting (ACE) » Angiotensin I Converting Enzyme-2, Recombinant, Rat (Ace-2)

Angiotensin I Converting Enzyme-2, Recombinant, Rat (Ace-2)

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Specifications

ACE-2, also called ACEH (ACE homologue), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE.1 The predicted rat ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity.2 Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of cardiovascular function in vivo.3 In addition, ACE-2 has a critical function in acute lung injury.
Catalog #A2295-05K
Molecular MassThe 733 amino acid residue rrACE-2 has a predicted molecular mass of approximately 85kD. By SDS-PAGE, the apparent molecular mass of the glycosylated protein is approximately 120kD under reducing and non-reducing conditions.
Biological Activity Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH.
Specific activity 300 pmoles/min/ug.
Endotoxin 1EU/ug of the enzyme as determined by the LAL method.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceThe ectodomain of the recombinant rat angiotensin I converting enzyme-2 was expressed as a secreted protein with a C-terminal 10-his tag in murine myeloma cells (NS0)
Purity95% as determined by SDS-PAGE and visualized by silver stain.
Concentration~0.5mg/ml
FormSupplied as a liquid in 12.5mM Tris, 0.1M NaCl, 0.5uM ZnCl2, pH 8.0, 50% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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