| Angiotensin II mediates its action by interacting with membrane receptor, Angiotensin II type 1 receptors. The signal is transmitted via G-proteins that activate a phosphatidylinositol- calcium second messenger system. ATII receptors are integral membrane proteins of approx. 359-363aa and predicted to contain at least 7 transmembrane domains. The N and C-terminus are predicted to extracellular and cytoplasmic, respectively. Three isoforms of ATII receptors have been cloned. In rat, type 1a (359aa) is expressed in the liver kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal and vascular smooth muscles. Rat Type 1b (also known as AT3; 359aa) is found in liver, kidney, and aorta. Type 1c (177aa) has been detected in brain, kidney, liver, spleen and lung. |
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| Catalog # | A2295-07S |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 1-10ug/ml (ECL). Detects ~45kD band. |
| ELISA: 1:10,000-1:100,000 using 50-100ng of A2295-07U control peptide/well. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 40% glycerol. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide consisting of 18aa sequence mapping near the C-terminus of human AT2 ( KLH). |
| Specificity | Recognizes human AT2 receptor. No sequence homology with other G-protein coupled receptors. Species Sequence Homology |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
