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You are here:Home » Antibodies » Abs to Receptors » Anti -Angiotensin II Type 1 Receptor (AT1)

Anti -Angiotensin II Type 1 Receptor (AT1)

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Specifications

Clone Host Grade Applications
Polyclonal Chicken Affinity Purified E B
Angiotensin II mediates its action by interacting with membrane receptor, Angiotensin II type 1 receptors. The signal is transmitted via G-proteins that activates a phosphatidylinositol- calcium second messenger system. ATII receptors are integral membrane proteins of approx.359-363aa and predicted to contain at least 7 transmembrane domains. The N and C-terminus are predicted to extracellular and cytoplasmic, respectively. Three isoforms of ATII receptors have been cloned. In rat type 1a (359 aa) is expressed in the liver kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal and vascular smooth muscles (1). Rat Type 1b (also known as AT3; 359 aa) is found in liver, kidney, and aorta (2). Type 1c (177 aa) has been detected in brain, kidney, liver, spleen and lung (3).
Catalog #A2295-08D
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-10ug/ml using ECL. Detects ~45-kD band.
ELISA: 1:10,000-1:100,000 using 50-100ng of A2295-08K control peptide/well.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken (HRP) Rb x Ch
I1906-12 IgY, Chicken (FITC) Rb x Ch
I1906-14 IgY, Chicken (AP) Rb x Ch
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgY
HostChicken
SourceRat
Concentration~1mg/ml
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide.
PurityPurified by affiinity chromatography.
ImmunogenA 10aa peptide sequence mapping near the N-terminus of rat AT II (KLH).
SpecificityRecognizes rat Angiotensin II Type 1 Receptor. Species sequence homology: mouse, rat, human, ovine, porcine, canine, chimp, chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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