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You are here:Home » Antibodies » Abs to Ion Channel » Anti -Anion Exchanger 1 (AE1)

Anti -Anion Exchanger 1 (AE1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2 and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times. It is able to mediate anion exchange by itself. AE1 protein (also know as band 3) is the most abundant protein in red cell membrane. AE1 gene has been cloned and characterized from a variety of species including mouse, rat, human and chicken.
Catalog #A2295-13A
ApplicationsSuitable for use in Western Blot and ELISA. Other applications have not been tested.
Recommended DilutionWestern Blot: 1-10ug/ml (ECL.) AE1 has a molecular weight of ~90-100kD. It aggregates easily forming dimers and multimers. It may also be proteolytically processed to smaller fragments of 60, 40 and 20kD.
ELISA: 1-10ug/ml. Using 1ug/ml A2295-13B4 control peptide per well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.5, 0.2% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to a 20aa sequence near the N-terminus of rat AE1 (KLH coupled).
SpecificityRecognizes rat Anion Exchanger 1 (AE1). No significant sequence homology with other AE or other proteins. Species Sequence Homology
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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