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You are here:Home » Antibodies » Abs to Ion Channel » Anti -Anion Exchanger 1 (AE1)

Anti -Anion Exchanger 1 (AE1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300 aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself.
AE1 protein (also know as band 3) is the most abundant protein in red cell membrane. AE1 gene has been cloned and characterized from a variety of species including mouse, rat, human, and chicken. Human AE1 gene (chromosome 17q21-qter) encodes for a protein of 911 aa (rat 927 aa; mouse 929 aa). Several isoforms of AE1 are also transcribed. AE1 is primarily expressed in red cells and kidney. AE1 is dimeric in solution. Defects in AE1/band3 gene are the cause of hereditary ovalocystosis (HO)-a disease associated with high degree of membrane rigidity.
Catalog #A2295-13B2
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000. AE1 is approx 90-100kD proteins. It aggregates easily forming dimers and multimers. It may also be proteolytically processed to smaller fragments of 60, 40, 20kD.
ELISA: 1:10,000-50,000. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Control PeptideA2295-13B4, Anion Exchanger 1, Rat, Control Peptide (AE1)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationNot determined
FormSupplied as a liquid, neat serum, 0.05% sodium azide, 40% glycerol.
PuritySerum
ImmunogenAn 20 AA Peptide sequence near the N-terminus of rat AE-1 (1). The peptide was coupled to KLH
SpecificityRecognizes rat Anion Exchanger 1 (AE1). Species sequence homology: mouse 90%; human 75%; bovine 70%. No significant sequence homology is detected with other AE or other proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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