| Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300 aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself. |
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| Catalog # | A2295-13B3 |
| AE1 protein (also know as band 3) is the most abundant protein in red cell membrane. AE1 gene has been cloned and characterized from a variety of species including mouse, rat, human, and chicken. Human AE1 gene (chromosome 17q21-qter) encodes for a protein of 911 aa (rat 927 aa; mouse 929 aa). Several isoforms of AE1 are also transcribed. AE1 is primarily expressed in red cells and kidney. AE1 is dimeric in solution. Defects in AE1/band3 gene are the cause of hereditary ovalocystosis (HO)-a disease associated with high degree of membrane rigidity. |
| Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1-10ug/ml using ECL. |
| ELISA: 0.5-1ug/ml Control peptide can be used to coat ELISA plates at 1ug/ml. |
| Immunohistochemistry: 2-10ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Rat |
| Concentration | 1mg/ml |
| Form | Supplied as a lyophilized powder from PBS, pH 7.4, 0.1% BSA. |
| Purity | Purified over the peptide-Sepharose column |
| Immunogen | An 20 AA Peptide sequence near the N-terminus of rat AE-1 (1). The peptide was coupled to KLH |
| Specificity | The 20 AA rat control peptide is 90% conserved in mouse, 75% in human and 70% in bovine AE1. No significant sequence homology is detected with other AE or other proteins. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
