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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum E B

Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300 aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself.
Human AE2 gene (chromosome 7q35-q36) encodes a protein of 124 aa (mouse 1237 aa, rat 1234aa). Several isoforms of AE2 are also expressed as a result of alternative splicing. AE2 is widely expressed in many tissues.
Catalog #	A2295-14D
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000-1:5000
	ELISA: 1:10,000-50,000
	Optimal dilutions to be determined by the researcher.
Control Peptide	A2295-14D1 Anion Exchanger 2, Rat Control Peptide
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	Not determined
Form	Supplied as a liquid in PBS and 0.05% sodium azide, 40% glycerol.
Purity	Serum
Immunogen	Synthetic peptide corresponding to a 21 AA Peptide sequence near the N-terminus of rat AE-2 (KLH).
Specificity	Recognizes rat AE-2. Species sequence homology: mouse- 100%, rabbit-90%, human-85%, chicken-80%.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.