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You are here:Home » Antibodies » Abs to Ion Channel » Anti -Anion Exchanger 2 (AE2)

Anti -Anion Exchanger 2 (AE2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300 aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself.
Human AE2 gene (chromosome 7q35-q36) encodes a protein of 124 aa (mouse 1237 aa, rat 1234aa). Several isoforms of AE2 are also expressed as a result of alternative splicing. AE2 is widely expressed in many tissues.
Catalog #A2295-14D
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000
ELISA: 1:10,000-50,000
Optimal dilutions to be determined by the researcher.
Control PeptideA2295-14D1 Anion Exchanger 2, Rat Control Peptide
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationNot determined
FormSupplied as a liquid in PBS and 0.05% sodium azide, 40% glycerol.
PuritySerum
ImmunogenSynthetic peptide corresponding to a 21 AA Peptide sequence near the N-terminus of rat AE-2 (KLH).
SpecificityRecognizes rat AE-2. Species sequence homology: mouse- 100%, rabbit-90%, human-85%, chicken-80%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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