Anti -Anion Exchanger 3 (AE3, Anion exchange protein 3, CAE3/BAE3, Cardiac/brain band
3-like protein, Neuronal band 3-like protein, SLC2C, Solute carrier family 4 member 3)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
E B |
|
| Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300 aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself. Human AE3 gene (chromosome 2q36) encodes a protein of 1233 aa (mouse/rat 1227 aa). AE3 is primarily expressed in excitable tissue such cardiac muscle, retina, and brain. | | | Catalog # | A2295-14F | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000-1:5000 (ECL) | | ELISA: 1:10,000-50,000. A2295-14F2 Control peptide can be used to coat ELISA plates at 1ug/ml. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Rat | | Concentration | Not Determined | | Form | Supplied as a liquid in PBS, 0.05% sodium azide. | | Purity | Serum | | Immunogen | Synthetic 20aa peptide sequence near the N-terminus, Cytoplasmic domain of rat AE-3 (KLH). Species sequence homology | | Specificity | Recognizes rat AE3. No significant sequence homology is detected with other AE or other proteins. Does not recognize cardiac AE3. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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