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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum E B

Anion exchangers (AE) are membrane proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself.
Recently, a new member of AE family, AE4, has been cloned from rabbit and human. Human AE4 (945aa; chromosome 5) is predicted to have 14 TM domains. It is strongly expressed in the kidney. Rabbit and human AE4 share 84% identity.
Catalog #	A2295-14G
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:10,000-50,000. Coat ELISA plates at 1ug/ml with A2295-14G2-Anion Exchanger 4, Control Peptide Human.
	Western Blot: 1:1000-1:5000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in PBS, 0.05% sodium azide, before the addition of glycerol to 40%.
Purity	Serum
Immunogen	Synthetic peptide corresponding to 15aa near the cytoplasmic C-terminus of human AE4 (KLH).
Specificity	Recognizes human AE4. Sequence Homology
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.