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You are here:Home » Antibodies » Abs to Ankyrin » Anti -Ankyrin B (RFXANK, Regulatory Factor X)

Anti -Ankyrin B (RFXANK, Regulatory Factor X)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
The cytoplasmic domains of many integral membrane proteins including ion channels and cell adhesion molecules are linked to the actin/spectrin cytoskeletal network by a family of proteins known as ankyrins. Ankyrin belongs to a family of closely related polypeptides associated with the plasma membrane of cells in a variety of cell types (e.g., lymphocytes, platelets, fibroblasts and endothelial cells) and various tissues (e.g., brain, kidney, muscle and many epithelial tissues). Ankyrin molecules contain three distinct domains: an NH2-terminal membrane-binding domain (~89-95kD), a spectrin binding domain (~62kD), and a COOH-terminal domain whose length varies as a result of alternative mRNA splicing. Sequences in the COOH-terminal domain are also the most variable between ankryin family members which include ankyrin R and ankyrin G. Ankryin B or brain-type ankyrin is expressed predominantly as 220kD and 440kD isoforms that are the product of a single gene. Ankyrin G is a marker of axon initial segments, nodes of Ranview, sites of cell-cell contact on epithelial cells and intracellular membranes of some cells.
Catalog #A2295-17
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 0.1-1.0ug/ml
Western Blot: 1.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3G7
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide derived from the human ankyrin B protein.
SpecificityRecognized human ankyrin B. It does not crossreact with ankyrin R or ankyrin G. Recognizes ankyrin B isoforms containing a spectrin binding domain. On Western blots, this antibody detects bands of approximately 440, 220 and 150kD. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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