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You are here:Home » Antibodies » Antibodies-Ankyrin » Anti -Ankyrin G (RFXANK, Regulatory Factor X)

Anti -Ankyrin G (RFXANK, Regulatory Factor X)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IF
Ankyrin G is found in several tissues including brain where, due to tissue-specific RNA processing, it is expressed as two different isoforms, localized to axon initial segments and nodes of Ranvier. Ankyrin G is also found at sites of epithelial cell- cell contact and in the intracellular membranes of some cells. Ankyrin G isoforms contain a unique stretch of sequence highly enriched in serine and threonine residues immediately following a globular head domain. Ankyrin G is thought to participate in the maintenance and targeting of ion channels and cell adhesion molecules to the nodes of Ranvier and to axon initial segments.
Catalog #A2295-18
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsELISA (Native): 0.1-1.0ug/ml
Western Blot: 1-3ug/ml. For best results on rat brain, mouse brain or MDCK cell lysates, add 0.3M sucrose to incubation buffer. Detects a band between ~200-300kD.
Immuno uorescence (Frozen): 5-10ug/ml. Sensitive to fixation conditions. Recommend 1% paraformaldehde for perfusion and 4 hours of post fixation, after perfusion. For sciatic nerve, recommend 2% paraformaldehyde for 2 hours at 4ºC.
Immunoprecipitation (Native): 5ug/ IP
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No3G8
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide derived from the spectrin-binding domain of the human ankyrin G protein.
SpecificityRecognizes human Ankyrin G. Does not crossreact with related ankyrin isoforms. Also recognizes all splice forms of Ankyrin G containing a spectrin-binding domain
Species CrossreactivityMouse, rabbit, rat and canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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