| Ankyrin G is found in several tissues including brain where, due to tissue-specific RNA processing, it is expressed as two different isoforms, localized to axon initial segments and nodes of Ranvier. Ankyrin G is also found at sites of epithelial cell- cell contact and in the intracellular membranes of some cells. Ankyrin G isoforms contain a unique stretch of sequence highly enriched in serine and threonine residues immediately following a globular head domain. Ankyrin G is thought to participate in the maintenance and targeting of ion channels and cell adhesion molecules to the nodes of Ranvier and to axon initial segments. |
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| Catalog # | A2295-18 |
| Applications | Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested. |
| Recommended Dilutions | ELISA (Native): 0.1-1.0ug/ml. |
| Western Blot: 1-3ug/ml. For best results on rat brain, mouse brain or MDCK cell lysates, add 0.3M sucrose to incubation buffer. Detects a band between ~200-300kD. |
| Immuno uorescence (Frozen): 5-10ug/ml. Sensitive to fixation conditions. Recommend 1% paraformaldehde for perfusion and 4 hours of post fixation, after perfusion. For sciatic nerve, recommend 2% paraformaldehyde for 2 hours at 4ºC. |
| Immunoprecipitation (Native): 5ug/ IP |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 3G8 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Synthetic peptide derived from the spectrin-binding domain of the human ankyrin G protein. |
| Specificity | Recognizes human Ankyrin G. Does not crossreact with related ankyrin isoforms. Also recognizes all splice forms of Ankyrin G containing a spectrin-binding domain |
| Species Crossreactivity | Mouse, rabbit, rat and canine. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
