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Anti-Nuclear Antibodies (ANA), Mouse, BioAssay™ ELISA Kit
Anti-Nuclear Antibodies (ANA), Mouse, BioAssay™ ELISA Kit
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| Catalog # | A2298-12J | | Principle of the Test | Anti-Nuclear Antibodies (ANA) ELISA kit is based on binding of ANA from serum samples to extracted nuclear antigen immobilized on microtiter wells. After a washing step, goat anti-mouse IgG-HRP conjugate is added. After another washing step, to remove all the unbound enzyme conjugate, chromogenic substrate (TMB) is added and color developed. The enzymatic reaction (blue color) is directly proportional to the amount of ANA present in the sample. The reaction is terminated by adding stopping solution (converts blue to yellow). Absorbance is then measured on a microtiter well ELISA reader at 450nm. The concentration of anti-ANA Ig’s (total) in samples and control is read off the standard curve. | | Detection limit | ~0.25ug/ml. The detection limit is defined as the value deviating by 2 SD from the zero standard. | | Intra-assay precision | 3.6-7.4%. | | Inter-assay precision | 7.8- 9.8%. | | Kit Components | | | A2298-12J1 | Microtiter Plate,1x96 wells | | A2298-12J2 | Standard 0ug/ml, 1x750ul | | A2298-12J3 | Standard 0.625ug/ml, 1x750ul | | A2298-12J4 | Standard 1.25ug/ml, 1x750ul | | A2298-12J5 | Standard 2.5ug/ml, 1x750ul | | A2298-12J6 | Standard 5ug/ml, 1x750ul | | A2298-12J7 | Standard 10ug/ml, 1x750ul | | A2298-12J8 | IgG (HRP),100X, 1x120ul | | A2298-12J9 | Sample Diluent 10X, 1x10ml | | A2298-12J10 | Wash Solution (100X), 1x10ml | | A2298-12J11 | TMB Substrate, 1x12 ml | | A2298-12J12 | Stop Solution, 1x12ml | | | Storage and Stability | | | Store all components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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