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Anti-Nuclear Antibodies (ANA), Mouse, BioAssay™ ELISA Kit
Anti-Nuclear Antibodies (ANA), Mouse, BioAssay™ ELISA Kit
Pricing
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| Catalog # | A2298-12J | | Principle of the Test | Anti-Nuclear Antibodies (ANA) ELISA kit is based on binding of mouse ANA IgG+IgA+IgM from serum samples to extracted nuclear antigen immobilized on microtiter wells. After a washing step, IgG (HRP) goat anti-mouse is added. After another washing step, to remove all the unbound enzyme conjugate, chromogenic substrate (TMB) is added and color developed. The enzymatic reaction (blue color) is directly proportional to the amount of ANA present in the sample. The reaction is terminated by adding stopping solution (converts blue to yellow). Absorbance is then measured on a microtiter well ELISA reader at 450nm. The acivity of total mouse antibodies in samples is calculated relative to mouse ANA calibrators. | | Kit Components | A2298-12J1: Microtiter Plate, 1x96 wells | | A2298-12J2: Standard 50U/ml, 1x650ul | | A2298-12J3: Standard 200U/ml, 1x650ul | | A2298-12J4: Standard 500U/ml, 1x650ul | | A2298-12J5: Standard 1000U/ml, 1x650ul | | A2298-12J8: IgG (HRP) 100X, 1x150ul | | A2298-12J9: Sample Diluent 20X, 1x10ml | | A2298-12J10: Wash Solution 100X, 1x10ml | | A2298-12J11: TMB Substrate, 1x12ml | | A2298-12J12: Stop Solution (1% H2SO4), 1x12ml | | A2298-12J13: Low NSB Sample Diluent 1x30ml | | Storage and Stability | Store all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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