| Anti-Nuclear Antibodies (ANA) are autoantibodies which binds to cellular nuclear antigens including ds-DNA, ss-DNA histones, ribonucleoproteins (RNP) and the SS-A, SS-B, and Sm antigens. Currently, ANA is widely used as screening procedure for autoantibodies. |
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| Catalog # | A2298-12M |
| The frequency of ANA positivity in various rheumatic diseases has been reported for SLE, rheumatoid arthritis (RA), progressive systemic sclerosis (PSS), polymyositis (PM), dermatomyositis (DM), mixed connective tissue diseases, drug-induced SLE, and Sjogren's syndrome (SS). Most of these studies are based on tedious immunofluorescence assay. Recently, ELISA has been used for this purpose. |
| Sample Type | Serum. Other fluids not optimized. |
| Intended Use | Semi-quantitative measurement of ANA in serum to further investigate the presence of specific autoantibodies. |
| Test Principle | Anti-Nuclear Antibodies (ANA) ELISA kit is based on binding of ANA from serum samples to extracted nuclear antigen immobilized on microtiter wells. After a washing step, goat anti-human IgG-HRP conjugate is added. After another washing step, to remove all the unbound enzyme conjugate, chromogenic substrate (TMB) is added and color developed. The enzymatic reaction (color) is directly proportional to the amount of ANA present in the sample. Adding stopping solution terminates the reaction. Absorbance is then measured on a microtiter well ELISA reader at 450nm and the concentration of ANA in samples is calculated as ANA index (AI) which is defined as the ratio of net absorbance of the test sample and net absorbance of the negative or endpoint-cutoff control. |
| Kit Components | Nuclear antigen coated microwell strips (96 wells), Ready-to-use |
| Prediluted ANA Endpoint Cutoff Control: 1X0.5ml |
| Prediluted ANA Negative Control: 1X0.35ml |
| Prediluted ANA Positive Control: 1X0.35ml |
| Sample Diluent: 1X25ml |
| Wash buffer (100X): 1X10ml (dilute 1:100 with distilled water) |
| Goat Anti-hIgG HRP Conjugate: 1X11ml |
| HRP Substrate Solution: 1X11ml |
| Stop Solution: 1X10ml |
| Storage and Stability | Store liquids at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial prior to removing the cap. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
