Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Protease Inhibitors » Anti -Antiplasmin, alpha2 (Alpha2-antiplasmin, SERPINF2, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2, A2AP, AAP, ALPHA-2-PI, API, PLI, alpha-2-antiplasmin, alpha-2-plasmin inhibitor, serine (or cysteine

Anti -Antiplasmin, alpha2 (Alpha2-antiplasmin, SERPINF2, serpin peptidase inhibitor, clade
F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2, A2AP, AAP,
ALPHA-2-PI, API, PLI, alpha-2-antiplasmin, alpha-2-plasmin inhibitor, serine (or cysteine

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
alpha 2Antiplasmin (alpha,2AP), also known as alpha 2Plasmin Inhibitor (alpha 2PI), is a member of the SERPIN family of proteinase inhibitors and the primary inhibitor of the enzyme plasmin in blood. It is produced in the liver and circulates in plasma at ~70ug/ml (~1uM). alpha 2AP is a single chain molecule with a mass of 67kD as determined by SDS-PAGE. The primary target enzyme for alpha 2AP is plasmin, but alpha 2AP also acts as secondary or “backup” inhibitor of activated F.XI, activated Protein C and trypsin. Inhibition of these enzymes by alpha 2AP occurs by proteolytic cleavage after Arg364 with subsequent rapid formation of a stable, inactive 1:1 enzyme-alpha 2AP complex. alpha 2AP also acts to regulate fibrinolysis by binding to the lysine binding sites on plasminogen thus competitively inhibiting plasminogen binding to fibrin. About 30% of alpha 2AP present in plasma is partially degraded and lacks a peptide in the carboxyl region that contains the plasminogen-binding site. This form of alpha 2AP (~65kD) has a reduced rate of plasmin inhibition and has been referred to as the “slow form” of alpha 2AP. During fibrin formation, a portion of circulating alpha 2AP is cross-linked to the alpha -chain of fibrin by activated factor XIII, and this linking of plasmin inhibitor to the plasmin substrate provides an additional measure of protection to the fibrin clot from proteolysis by plasmin.
Catalog #A2298-13M
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:16000
Western Blot: 0.3-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostGoat
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to human Alpha2-antiplasmin.
SpecificityRecognizes human Alpha2-antiplasmin at ~70kD in Human Duodenum and Ileum lysates (calculated MW of 54.6kD according to NP_000925.2). The observed MW corresponds to earlier findings in literature with different antibodies (Lee et al, Blood. 2004 May 15;103(10)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links