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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

alpha 2Antiplasmin (alpha,2AP), also known as alpha 2Plasmin Inhibitor (alpha 2PI), is a member of the SERPIN family of proteinase inhibitors and the primary inhibitor of the enzyme plasmin in blood. It is produced in the liver and circulates in plasma at ~70ug/ml (~1uM). alpha 2AP is a single chain molecule with a mass of 67kD as determined by SDS-PAGE. The primary target enzyme for alpha 2AP is plasmin, but alpha 2AP also acts as secondary or “backup” inhibitor of activated F.XI, activated Protein C and trypsin. Inhibition of these enzymes by alpha 2AP occurs by proteolytic cleavage after Arg364 with subsequent rapid formation of a stable, inactive 1:1 enzyme-alpha 2AP complex. alpha 2AP also acts to regulate fibrinolysis by binding to the lysine binding sites on plasminogen thus competitively inhibiting plasminogen binding to fibrin. About 30% of alpha 2AP present in plasma is partially degraded and lacks a peptide in the carboxyl region that contains the plasminogen-binding site. This form of alpha 2AP (~65kD) has a reduced rate of plasmin inhibition and has been referred to as the “slow form” of alpha 2AP. During fibrin formation, a portion of circulating alpha 2AP is cross-linked to the alpha -chain of fibrin by activated factor XIII, and this linking of plasmin inhibitor to the plasmin substrate provides an additional measure of protection to the fibrin clot from proteolysis by plasmin.
Catalog #	A2298-13P
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:10,000
	Western Blot: 1-5ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Mouse
Concentration	As reported
Form	Supplied as a liquid in 0.05M sodium phosphate, pH 6.6, 0.1M sodium chloride, 1mM EDTA.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Recombinant mouse alpha-2-antiplasmin.
Specificity	Recognizes mouse Alpha 2 Antiplasmin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.