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You are here:Home » Antibodies » Abs to Coagulation Factors » Anti -Antitrypsin Activated Protein C (APC-AAT complexes, alpha1 Proteinase Inhibitor, alpha1 PI, native and denatured)

Anti -Antitrypsin Activated Protein C (APC-AAT complexes, alpha1 Proteinase Inhibitor,
alpha1 PI, native and denatured)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E
Protein C is a vitamin K-dependent serine protease produced in the liver and made up of 2 polypeptide chains. The 62kD proenzyme is activated by thrombin and the active enzyme cleaves factor Va and VIIIa and thus inhibits blood coagulation. The molecular weight of the active enzyme is 55kD and the normal concentrations in human plasma is approximately 1-3ng/ml because of the very fast turnover, the proenzyme concentration is approximately 3ug/ml. The activated protein C (APC) is inhibited by members of the serine protease inhibitor (serpin) family, of which a1-antitrypsin (AAT) and protein C inhibitor (PCI) are the most important.
Catalog #A2298-38C
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionELISA: 1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No7
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
PurityPurified by Protein A/G affinity chromatography from culture supernatant.
ImmunogenRecombinant form of human activated protein C (Drotrecogin alfa, Xigris)
SpecificityHas specificity for a conformation-dependent neoepitope that is expressed in activated protein C upon complex-formation with a1-antitrypsin. No reaction is seen to non-complexed a1-antitrypsin and only very little cross reaction to protein C zymogen. Note that specificity is calcium dependent.
Reactivity
Reacts strongly with APC-AAT complexes in ELISA. It can be used in sandwich ELISA in combination with a polyclonal anti-protein C antiserum. Note that the conformational neoepitope expressed in the APC-AAT complex can also be expressed in APC coated directly onto a high-binding microtiter plate.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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