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You are here:Home » Antibodies » Abs to Coagulation Factors » Anti -a1-Antitrypsin (AAT, alpha-1-AT, alpha1 Proteinase Inhibitor, alpha1 PI) (Biotin)

Anti -a1-Antitrypsin (AAT, alpha-1-AT, alpha1 Proteinase Inhibitor, alpha1 PI) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Highly Purified E B IH Gel
Alpha-1-antitrypsin (AAT) consists of a single polypeptide chain and has a molecular weight of 51kD. It exists in a number of genetic variants. MM variant is the most common. Alpha-1-AT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leukocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung. In the absence of AAT, these enzymes are not inhibited and they may digest pulmonary parenchyma. AAT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn. Increase in AAT occurs as an acute phase response to tissue necrosis and inflammation. Serum level of alpha-1-AT is elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis. In colorectal and lung carcinomas, AAT has also related to their invasive and metastatic capacity. The average level of AAT in plasma is 1.3g/L.
Catalog #A2298-39
ApplicationsSuitable for Western Blot, Dot Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications have not been tested.
Recommended DilutionELISA: 1:20,000-1:100,000
Western Blot: 1:2000-1:10,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~10mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease Free), 0.01% sodium azide. Labeled with Biotin.
PurityPuri ed by delipidation, salt fractionation and ion exchange chromatography.
ImmunogenAlpha-1-anti-Trypsin from human plasma.
SpecificityRecognizes human Alpha-1-anti-Trypsin. Assay by immunoelectrophoresis resulted in a single precipitin arc against puri ed and partially puri ed alpha-1-anti-trypsin from human plasma, anti-goat serum and anti-biotin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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