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You are here:Home » Molecular Biology » MB-Proteins » AP2m1 (Adaptor-related Protein Complex 2 mu 1, Clathrin Assembly Protein Complex 2) Recombinant, Human

AP2m1 (Adaptor-related Protein Complex 2 mu 1, Clathrin Assembly Protein Complex 2)
Recombinant, Human

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Specifications

AP2M1 is a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. This protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. It may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein.
Catalog #A2298-51H
DescriptionRecombinant Human AP2M1 produced in E.Coli is a non-glycosylated, polypeptide chain containing amino acids (1-435) and having a total molecular mass of 50kD. Recombinant AP2M1 contains T7 tag at N-terminus and is purified by proprietary chromatographic techniques.
Applications ELISA MS Western Blotting
StabilityRecombinant AP2M1 although stable at 140C for 1 week, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Molecular Weight50kD
SourceE. coli
Purity95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration~0.1mg/ml
FormSupplied as a liquid in 10mM Tris, pH 8.0, 0.1% Triton X-100 and 0.002% sodium azide.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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