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You are here:Home » Molecular Biology » MB-All Other » APAAP, Mouse (Alkaline Phosphatase-Anti-Alkaline Phosphatase Complex)

APAAP, Mouse (Alkaline Phosphatase-Anti-Alkaline Phosphatase Complex)

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Specifications

APAAP complex is prepared from a mixture of purified alkaline phosphatase (calf intestine) and purified mouse monoclonal anti-alkaline phosphatase antibody
Catalog #A2298-57
ApplicationsSuitable for use in ELISA, Immunocytochemistry, Histochemistry and Immunoblot. Other applications not tested.
Recommended DilutionELISA: 1:1000-1:4000
Immunocytochemistry: 1:50-1:100
Immunohistochemistry: 1:50-1:100
Immunoblot: 1:200-1:500
Optimal dilution to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
FormSupplied as a liquid in 50 mM Tris-HCl, 150 mM NaCl, 1 mM MgCl2, pH 7.5, containing 50% glycerol, 1% BSA, and 0.05% sodium azide.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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