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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP IH

The sequence-specific transcription factor activator protein 2 alpha (AP-2 alpha) is required for normal growth and morphogenesis during mammalian development (1,2). Decreased or loss of AP-2 alpha expression has been observed in many different types of human cancers including breast cancer (3,4), ovarian cancer (5), melanoma (6) and prostate cancer (7). These findings suggest that AP-2alpha expression plays a crucial role in tumorigenicity. Studies have also shown that p53 overexpression in human breast carcinoma cells induces the level of AP-2 alpha expression. Furthermore, p53 binds to the cis-element in the AP-2alpha promoter, suggesting that AP-2 alpha is a target of p53. AP-2 alpha may mediate the effect of p53 to inhibit cell proliferation (8).
Catalog #	A2298-59D
Applications	Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western blot: 1:1000
	Immunoprecipitation: 1:50
	Immunohistochemistry (Paraffin): 1:300
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	As reported
Form	Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol.
Purity	Purified by protein A and peptide affinity chromatography
Immunogen	Synthetic peptide (KLH-coupled) derived from a sequence of human AP-2 alpha.
Specificity	Detects endogenous levels of total human AP-2 alpha protein. Species Ccrossreactivity: mouse, rat and monkey.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.