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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -AP2m1 (Adaptor-related Protein Complex 2 mu 1)

Anti -AP2m1 (Adaptor-related Protein Complex 2 mu 1)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IH FC
Clathrin-coated vesicles, involved in endocytosis and Golgi processing, have a surface lattice containing clathrin triskelia and stoichiometric amounts of additional components termed "assembly proteins," or APs. The AP form at the plasma membrane, AP2, is composed of two large subunits of 100-115kDa, denoted AP2 alpha and AP2 beta, a medium chain of 50kD, designated AP50, and a small chain (1). The medium chain AP2 mu1 interacts specifically with the tyrosine-based signals of several integral membrane proteins through the consensus sequence YXXPhi, where X can be any residue and Phi is a large hydrophobic residue (2). Phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P(2)] is an important factor for a variety of cellular functions ranging from cell signaling to actin cytoskeletal dynamics and endocytic membrane traffic. It has been shown that the clathrin adaptor complex AP-2 as a regulator of phosphatidylinositol 4-phosphate 5-kinase (PIPK)-mediated PI(4,5)P(2) synthesis. AP-2 directly interacts with the kinase core domain of type I PIPK isozymes via its mu2-subunit in vitro and in native protein extracts (3).
Catalog #A2298-60A
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000 1: 5000
Immunohistochemistry: 1:100-1:250
Flow Cytometry: 1:60
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E142
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human AP2 mu1.
SpecificityRecognizes human AP2 Transcription Factor mu1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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