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You are here:Home » Molecular Biology » MB-Enzymes, Restriction » ApaI



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5'-G G G C C^C-3'
3'-C^C C G G G-5'
Catalog #A2298-63
Concentration 10u/ul
Source Acetobacter pasteurianus
Buffer 10mM Tris-HCl (pH 7.5), 10mM MgCl2 and 0.1mg/ml BSA. Incubate at 37°C. (Incubation at 30°C results in a 2-fold increase in activity.)
Diluent Buffer 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods–the Storage Buffer should be used.
Storage Buffer 10mM Tris-HCl (pH 7.4 at 25°C), 50mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Overdigestion Assay No detectable change in the specific fragmentation pattern is observed after 320-fold overdigestion (20u/ug lambda DNA x 16 hours) with ApaI.
Ligation/Recutting Assay After 50-fold overdigestion (3u/ug DNA x 17 hours) with ApaI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.02uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) Assay No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Blue/White Cloning Assay pUC57 was digested at a unique site with 10units of enzyme for 16 hours. After religation and transformation 0.3% of white colonies were detected.
Methylation Effects Sequence GGGCCm5C is cleaved approximately 150 times slower than the unmethylated one. Overlapping Dcm or CG methylation may influence DNA cleavage.
Stability during Prolonged Incubation A minimum of 0.2units of enzyme is required for complete digestion of 1ug of DNA in 16 hours at 37°C.
Thermal Inactivation Enzyme is inactivated by incubation at 65°C for 20min.
Digestion of Agarose-embedded DNA A minimum of 5units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Compatible Ends GGGCC^C–BseSI, GGGCC^C–Eco24I, GGGCC^C–SduI
Number of Recognition Sites in DNA
Lambda 1
PhiX174: 0
M13mp18/19: 0
pBR322: 0
pUC18/19: 0
pUC57: 1
pTZ19R/U: 0
pBluescriptIIKS(-/+): 1
pBluescriptIISK(-/+): 1
pACYC177: 0
pACYC184: 0
Unit Definition One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA-CpoI fragments in 1 hour at 37°C in 50ul of assay buffer.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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