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You are here:Home » Antibodies » Abs to Apoptosis » Anti -Apaf-1, CT (Apoptosis Protease Activating Factor-1)

Anti -Apaf-1, CT (Apoptosis Protease Activating Factor-1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1 (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 transcript is ubiquitously expressed in human tissues (1).
Catalog #A2298-64
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:2,000
Optimal dilution determined by the researcher.
Positive ControlHuman heart tissue lysate; 130kD band should be detected.
Related ProductsBlocking peptide (A2298-64P, 50ug/250ul) is available for competition studies.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenPeptide corresponding to amino acids 1,158 to 1,177 of human Apaf1. The sequence of the immunogenic peptide differs from that of murine Apaf1 by one amino acid.
SpecificityRecognizes human Apaf-1 at 130kD. Species Crossreactivity: Mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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