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You are here:Home » Antibodies » Abs to Apoptosis » Anti -APAF1 (Apoptotic Protease-activating Factor 1, APAF-1, KIAA0413)

Anti -APAF1 (Apoptotic Protease-activating Factor 1, APAF-1, KIAA0413)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
During apoptosis, Apaf-1 (apoptosis protease activating factor 1) binds to cytochrome c (Apaf-2), a reaction that requires dATP, and this leads to the formation of a large multimeric complex. The complex recruits pro-caspase-9, and activates caspase-9 (Apaf-3). Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf-1 is ubiquitously expressed in human tissues.
Catalog #A2298-64Z1
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.01% thimerosal, 30% glycerol.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa1161 from human Apaf-1.
SpecificityRecognizes human Apaf-1. Species Crossreactivity: mouse, rat, bovine, rabbit, monkey, equine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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