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You are here:Home » Antibodies » Abs to Apoptosis » Anti -Apaf-1, NT (Apoptosis Protease Activating Factor-1)

Anti -Apaf-1, NT (Apoptosis Protease Activating Factor-1)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IH IC
Apaf-1, or apoptotic protease activating factor 1, is a human protein homolog of the C. elagans apoptotic regulatory protein CED-4 that activates caspase-9 during apoptosis (1). Cytosolic Apaf-1 forms an oligomeric complex with caspase-9 in the presence of cytochrome c and dATP, which leads to caspase-9 activation and subsequent activation of caspase-3 (2, 3). Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains, consequently enhancing the catalytic activity of caspase-9 (4), leading to activation of the caspase signaling cascade and apoptosis.
Catalog #A2298-65Q
ApplicationsSuitable for use in Western Blotting, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blotting: 1:500
Immunohistochemistry: 1:50-1:100
Immunocytochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k25
HostRabbit
SourceHuman
FormSupplied as a liquid
PuritySupernatant
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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