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You are here:Home » Antibodies » Abs to Apoptosis » Anti -Apaf-1 (Apoptotic Peptidase Activating Factor 1, Apoptotic Protease-activating Factor 1, APAF 1, APAF1, APAF-1, CED4, DKFZp781B1145, KIAA0413)

Anti -Apaf-1 (Apoptotic Peptidase Activating Factor 1, Apoptotic Protease-activating
Factor 1, APAF 1, APAF1, APAF-1, CED4, DKFZp781B1145, KIAA0413)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum IH
Apaf-1, a 135kD member of NOD family proteins, is a mammalian homolog of C.elegans CED4. It consists of an N-terminal caspase recruitment domain (CARD), a central nucleotide-binding oligomerization domain (NOD) and multiple WD40 repeats at the C-terminus. Apaf-1 exists as an inactive protein in the cells and requires ATP for activation. It binds with cytochrome C and dATP and oligomerizes into a large apoptosome complex responsible for the activation of procaspase-9. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Apaf-1 is an essential downstream effector of p53-mediated apoptosis and is inactivated in metastatic melanomas.
Catalog #A2298-68D
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Positive ControlTestis
Cellular Localization
Cytoplasmic
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 0.05% sodium azide.
PuritySerum
ImmunogenSynthetic peptide corresponding to C-terminus human Apaf 1.
SpecificityRecognizes human Apaf 1. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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