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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

This gene encodes a tumor suppressor protein that includes among its many intracellular functions one of nuclear export. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.
Catalog #	A2298-70A
Applications
	Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions	Immunohistochemistry: Acetone fixed, frozen sections.
	Optimal dilution to be determined by the researcher.
Clinical Relevance	Detection of APC mutants from colon cell lines.
Positive Control	Colon cell line HCT116, SW480 and SW837 extracts.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	N/A
Clone Type	Monoclonal
Isotype	IgG1
Clone No	4i295
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS. No preservatives added.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Synthetic peptide corresponding to aa1-433 of the N-terminal fragment of human APC (Adenomutons Polyposis Coli gene Chr 5q) (coupled to maltose binding protein). Cellular Localization: Cytoplasmic and nuclear.
Specificity	Recognizes the N-terminal region of the human APC protein. Epitope Mapping
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.