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You are here:Home » Antibodies » Abs to Proteins » Anti -APC (Adenomatous Polyposis coli)

Anti -APC (Adenomatous Polyposis coli)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IH
APC gene product is associated with structural components of intracellular junctions, including conductin, and it competes with E-cadherin for binding to specific internal regions of both b- and g-catenin. Like APC, APC2 contains SAMP domains, which are required for conductin binding. Both APC and APC2 regulate the formation of active b-catenin-Tcf complexes.
Catalog #A2298-70B
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Formalin/paraffin): 1:25 for 60min. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min. followed by cooling at room temperature for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlColon carcinoma
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.2% BSA and 15mM sodium azide before the addition of glycerol to 40%.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide derived from C-terminus of human APC protein
Cellular LocalizationCytoplasmic
SpecificityRecognizes human APC, Mr 300kD
Species CrossreactivityMouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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