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You are here:Home » Antibodies » Antibodies-Proteins » Anti -APC, CT (Adenomatous Polyposis coli)

Anti -APC, CT (Adenomatous Polyposis coli)


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Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
APC is a ubiquitous, tumor-suppressor protein that is mutated in most colon cancers, generally resulting in a gene product that is truncated in the carboxy terminal region. APC contains several binding domains. The region used as an immunogen overlaps the EB1, DLG (PDZ sequence) and PSD-93 domains. These domains bind microtubules, mitotic spindles and F-actin and are involved in cell cycle, division and migration. The immunogen does not include the beta-catenin-binding region, which antagonizes Wnt signaling. The human APC amino acid sequence used as immunogen is 83% and 86% identical to mouse and rat APC, respectively.
Catalog #A2298-70C
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and ELISA. Optimal dilutions should be determined by each laboratory for each application.
Recommended DilutionsWestern Blot: 0.1-0.2ug/ml, the detection limit is~1ng/lane under non-reducing and reducing conditions.
Immunohistochemistry: 5-15ug/ml
ELISA: 0.5-1ug/ml, the deterction limit is ~0.2ng/well.
Optimal dilutions to be determined by researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 500ul sterile PBS, 40-50% glycerol.
PurityPurified by Immunoaffinity chromatography.
ImmunogenE. coli-derived rhAPC C-Terminus (aa25372843)
SpecificityRecognize human APC C-Terminus.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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