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You are here:Home » Antibodies » Abs to Proteins » Anti -APC, CT (Adenomatous Polyposis coli)

Anti -APC, CT (Adenomatous Polyposis coli)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
APC is a ubiquitous, tumor-suppressor protein that is mutated in most colon cancers, generally resulting in a gene product that is truncated in the carboxy terminal region. APC contains several binding domains. The region used as an immunogen overlaps the EB1, DLG (PDZ sequence) and PSD-93 domains. These domains bind microtubules, mitotic spindles and F-actin and are involved in cell cycle, division and migration. The immunogen does not include the beta-catenin-binding region, which antagonizes Wnt signaling. The human APC amino acid sequence used as immunogen is 83% and 86% identical to mouse and rat APC, respectively.
Catalog #A2298-70C
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and ELISA. Optimal dilutions should be determined by each laboratory for each application.
Recommended DilutionsWestern Blot: 0.1-0.2ug/ml, the detection limit is~1ng/lane under non-reducing and reducing conditions.
Immunohistochemistry: 5-15ug/ml
ELISA: 0.5-1ug/ml, the deterction limit is ~0.2ng/well.
Optimal dilutions to be determined by researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 500ul sterile PBS, 40-50% glycerol.
PurityPurified by Immunoaffinity chromatography.
ImmunogenE. coli-derived rhAPC C-Terminus (aa25372843)
SpecificityRecognize human APC C-Terminus.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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