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Anti -APC, CT (Adenomatous Polyposis coli)
Anti -APC, CT (Adenomatous Polyposis coli)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B IH |
|
| APC is a ubiquitous, tumor-suppressor protein that is mutated in most colon cancers, generally resulting in a gene product that is truncated in the carboxy terminal region. APC contains several binding domains. The region used as an immunogen overlaps the EB1, DLG (PDZ sequence) and PSD-93 domains. These domains bind microtubules, mitotic spindles and F-actin and are involved in cell cycle, division and migration. The immunogen does not include the beta-catenin-binding region, which antagonizes Wnt signaling. The human APC amino acid sequence used as immunogen is 83% and 86% identical to mouse and rat APC, respectively. | | | Catalog # | A2298-70C | | Applications | Suitable for use in Western Blot, Immunohistochemistry and ELISA. Optimal dilutions should be determined by each laboratory for each application. | | Recommended Dilutions | Western Blot: 0.1-0.2ug/ml, the detection limit is~1ng/lane under non-reducing and reducing conditions. | | Immunohistochemistry: 5-15ug/ml | | ELISA: 0.5-1ug/ml, the deterction limit is ~0.2ng/well. | | Optimal dilutions to be determined by researcher. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | ~0.2mg/ml | | Form | Supplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 500ul sterile PBS, 40-50% glycerol. | | Purity | Purified by Immunoaffinity chromatography. | | Immunogen | E. coli-derived rhAPC C-Terminus (aa2537–2843) | | Specificity | Recognize human APC C-Terminus. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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