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Anti -APC2, NT (Adenomatous Polyposis coli Tumor Suppressor Protein 2, APCL)
Anti -APC2, NT (Adenomatous Polyposis coli Tumor Suppressor Protein 2, APCL)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| The adenomatous polyposis coli tumor suppressor protein homologue APC2 (formerly known as APCL) is involved in the p53/Bcl-2-linked pathway of cell-cycle progression and cell death. APC2 is also heavily involved in the regulation of beta-Catenin transcriptional activity and the formation of beta-Catenin-TCF complexes, and as thus, may also be required for Wnt transduction in all cells. Loss of function mutations in one of the two human APC genes result in improper activation of the Wnt signaling pathway, and are one of the first steps in the development of colon cancer, as well as other tumors. Current research also speculates that APC2 interacts | | | with and may also regulate the cytoskeleton. APC2 bears significant sequence homology to the tumor suppressor APC, and is expressed strongly and specifically in the central nervous system. The expression pattern of APC2 indicates a specific role in proliferation and differentiation in the central nervous system. APC2 is also expressed in several non-neuronal tissues and cell lines including breast, colon and ovary. Overexpressed human APC2 localizes to the perinuclear region and to some microtubles in the cell body. Endogenus human APC2 is diffusely distributed in the cytoplasm and co-localizes with both the Golgi apparatus and actin filaments, suggesting that it could have a role in cell motility or adhesion through interaction with actin filaments. | | | Catalog # | A2298-70E | | Applications | Suitable for use in Western Blot. Other applications have not been tested. | | Recommended Dilutions | Western Blot: 1-3ug/ml | | Optimal dilutions to be determined by the researcher. | | Positive Controls | Human A549, CaCo2, SW480, MCF-7 and T47D cell lysates. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.25mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide derived from the N-terminal region of the human APC2 protein. | | Specificity | Recognizes human adenomatous polyposis coli tumor suppressor protein homologue APC2 (formerly known as APCL). On Western Blot, it identifies one band at ~210kD and two doublet bands representing isoforms of breakdown products at ~120-140kD and ~80-95kD. Species Sequence Homology: mouse and rat | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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