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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -ANAPC10, NT (Anaphase-promoting Complex Subunit 10, APC10, Cyclosome Subunit 10 APC10)

Anti -ANAPC10, NT (Anaphase-promoting Complex Subunit 10, APC10, Cyclosome Subunit 10
APC10)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
APC is a ubiquitin ligase which specifically targets mitotic regulatory factors such as Pds 1/Cut 2 and cyclin B. It was found that APC 10/Doc 1 is localized in centrosomes and mitotic spindles throughout mitosis, while it is also localized in kinetochores from prophase to anaphase and in mid body in telophase and cytokinesis. These results strongly support the notion that human APC 10/Doc 1 may be one of the APC core subunits rather than the transiently associated regulatory factor.
Catalog #A2298-72Y1
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 2ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to mouse ANAPC10 at N-terminal.
SpecificityRecognizes mouse ANAPC10. Species sequence homology: human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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