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You are here:Home » Antibodies » Abs to Enzymes, Endonuclease » Anti -APE (Ref-1, Apurinic Apyrimidinic Endonuclease, APEX, APE-1, HAP1)

Anti -APE (Ref-1, Apurinic Apyrimidinic Endonuclease, APEX, APE-1, HAP1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IH
The mammalian apurinic/apyrimidinic endonuclease
(APE/ref-1) is responsible for the repair of AP sites in DNA. In addition, this enzyme functions as a redox factor facilitating the DNA binding capability of FOS, JUN, NfkB, HIF-1 alpha, Pax-5, Pax-8 and other transcription factors. APE/ref-1 has also been shown to control p53 activity through redox alteration. APE is linked to apoptosis, associated with thioredoxin, and altered levels of APE/ref-1 have been found in some cancers. APE appears to form a unique link between the DNA base excision pathway, oxidative signaling, transcription regulation, cancer and cell-cycle control.
Catalog #A2298-74
ApplicationsSuitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended DilutionWestern Blot: > 1:2000 Detects single 37kD band using ECL. Immunohistochemistry (frozen and paraffin fixed sections and cytospin preps): > 1:5000, it can be competitively inhibited from recognizing APE/ref-1 antigen in tissues using APE/ref-1 protein.
Optimal dilution determined by the researcher.
Positive ControlHeLa whole cell extract
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No3C107
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
PurityAscites
ImmunogenPurified human APE/ref protein. Epitope: amino acids 80-100.
Cellular LocalizationIn Immunohistochemistry, punctate nuclear staining is observed in most cell types. Cytoplasmic staining is seen in hepatocytes, large neurons and granule cells.
SpecificityRecognizes human APE/ref-1 protein. Species Crossreactivity: Human, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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