Anti -APE (Ref-1, Apurinic Apyrimidinic Endonuclease, APEX, APE-1, HAP1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Ascites |
B IH |
|
| The mammalian apurinic/apyrimidinic endonuclease | | | (APE/ref-1) is responsible for the repair of AP sites in DNA. In addition, this enzyme functions as a redox factor facilitating the DNA binding capability of FOS, JUN, NfkB, HIF-1 alpha, Pax-5, Pax-8 and other transcription factors. APE/ref-1 has also been shown to control p53 activity through redox alteration. APE is linked to apoptosis, associated with thioredoxin, and altered levels of APE/ref-1 have been found in some cancers. APE appears to form a unique link between the DNA base excision pathway, oxidative signaling, transcription regulation, cancer and cell-cycle control. | | | Catalog # | A2298-74 | | Applications | Suitable for use in Western Blot and Immunohistochemistry. | | Other applications not tested. | | Recommended Dilution | Western Blot: > 1:2000 Detects single 37kD band using ECL. Immunohistochemistry (frozen and paraffin fixed sections and cytospin preps): > 1:5000, it can be competitively inhibited from recognizing APE/ref-1 antigen in tissues using APE/ref-1 protein. | | Optimal dilution determined by the researcher. | | Positive Control | HeLa whole cell extract | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 3C107 | | Host | Mouse | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide. | | Purity | Ascites | | Immunogen | Purified human APE/ref protein. Epitope: amino acids 80-100. | | Cellular Localization | In Immunohistochemistry, punctate nuclear staining is observed in most cell types. Cytoplasmic staining is seen in hepatocytes, large neurons and granule cells. | | Specificity | Recognizes human APE/ref-1 protein. Species Crossreactivity: Human, mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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