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You are here:Home » Antibodies » Antibodies-Enzymes, Endonuclease » Anti -APE (Ref-1, Apurinic Apyrimidinic Endonuclease, APEX, APE-1, HAP1)

Anti -APE (Ref-1, Apurinic Apyrimidinic Endonuclease, APEX, APE-1, HAP1)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH IC IF N
The mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is responsible for the repair of AP sites in DNA. In addition, this enzyme has been shown to function as a redox factor facilitating the DNA binding capability of FOS, JUN, NfkB, HIF-1a as well as other transcription factors. Recently, APE has also been shown to control p53 activity through redox alteration. APE is also linked to apoptosis, associated with thioredoxin, and altered levels of APE have been found in some cancers (3). APE appears to form a unique link between the DNA base excision pathway, oxidative signaling, transcription regulation, cancer and cell-cycle control (4).
Catalog #A2298-74A
ApplicationsSuitable for use in Western Blot, Simple Western, Immunohistochemistry, Immunoprecipitation, Chromatin Immunoprecipitation, Blocking/Neutralization and Immunofluorescence/Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000 (ECL). Detects a single band at 37kD.
Simple Western: 1:12.5
Immunohistochemistry (Frozen/Paraffin): 1:100
Immunofluorescence (IC): 1:50-1:200
Immunoprecipitation: 7ug/ml
Chromatin Immunoprecipitation: 1:10-1:500
Blocking/Neutralization: 50-200 molar excess
Optimal dilutions to be determined by researcher.
Positive Control HeLa whole cell extract
Cellular LocalizationIn immunohistochemical experiments punctate nuclear staining is seen in most cell types. However, in hepatocytes, large neurons and granule cells, cytoplasmic staining is observed.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in Tris-glycine, 150mM NaCl, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenAffinity purified human Apurinic/Apyrimidinic Endonuclease (APE/ref-1) fusion protein.
SpecificitySpecific to the human APE/ref-1protein. Competitively inhibited from recognizing the APE/ref-1 antigen in tussues using APE/ref-1 prptein. Inhibits repair activity of ape protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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