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You are here:Home » Antibodies » Abs to Enzymes, Endonuclease » Anti -APE (Ref-1, Apurinic Apyrimidinic Endonuclease, APEX, APE-1, HAP1)

Anti -APE (Ref-1, Apurinic Apyrimidinic Endonuclease, APEX, APE-1, HAP1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
The mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is responsible for the repair of AP sites in DNA. In addition, this enzyme has been shown to function as a redox factor facilitating the DNA binding capability of FOS, JUN, NfkB, HIF-1a as well as other transcription factors. Recently, APE has also been shown to control p53 activity through redox alteration. APE is also linked to apoptosis, associated with thioredoxin, and altered levels of APE have been found in some cancers (3). APE appears to form a unique link between the DNA base excision pathway, oxidative signaling, transcription regulation, cancer and cell-cycle control (4).
Catalog #A2298-74A
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Apoptosis. Other applications not tested.
Recommended DilutionWestern Blot: 1:500 (ECL). Detects a single band at 37kD. Diluted antibody can be reused 1-3 times in Western Blot analysis.
Immunohistochemistry (Frozen, fixed paraffin, cytospin preps): 1:50.
Apoptosis: Can be used following the apoptosis (TUNEL) procedure using the Boehringer-Mannheim TUNEL assay kit (2). Antibody staining should be performed AFTER the TUNEL assay.
Optimal dilutions to be determined by researcher.
Positive Control HeLa whole cell extract.
Cellular LocalizationIn Immunohistochemistry, punctate nuclear staining is seen in most cell types. In hepatocytes, large neurons and granule cells, cytoplasmic staining is observed.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.05mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, before the addition of glycerol to 40%.
PurityPurified by immunoaffinity chromatography.
ImmunogenAffinity purified human Apurinic/Apyrimidinic Endonuclease (APE/ref-1) fusion protein.
SpecificitySpecific to the human APE/ref-1protein. Competitively inhibited from recognizing the APE/ref-1 antigen in tussues using APE/ref-1 prptein. Inhibits repair activity of ape protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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