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You are here:Home » Antibodies » Abs to Enzymes, Endonuclease » Anti -APEX1 (DNA-(Apurinic or Apyrimidinic Site) Lyase, APEX Nuclease, APEN, Apurinic-apyrimidinic Endonuclease 1, AP Endonuclease 1, APE-1, REF-1, Redox Factor-1, APE, APE1, APEX, APX, HAP1, REF1)

Anti -APEX1 (DNA-(Apurinic or Apyrimidinic Site) Lyase, APEX Nuclease, APEN,
Apurinic-apyrimidinic Endonuclease 1, AP Endonuclease 1, APE-1, REF-1, Redox Factor-1,
APE, APE1, APEX, APX, HAP1, REF1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
Reactive oxygen species (H2O2·, OH·, O2, HOCL) generated during normal cellular activities or by ionizing radiation and by many oxidizing/alkylating agents can induce a wide variety of lesions in DNA ranging from oxidized bases (8-oxoguanine and thymine glycol), base loss and DNA strand breaks with modified ends such as 3'-phosphate and 3'-phopshoglycolate. Unrepaired damaged/modified DNA has been implicated in aging and cancer. Many proteins that are involved in BER (base excision repair) have been identified in E. coli and mammals. Repair of modified is initiated by their removal by specific DNA glycosylase resulting in AP (apurinic/apyrimidinic) sites. A series of steps starting with the cleavage of the DNA strand adjacent to AP sites, removal of the deoxyribose phosphate residue, followed by gap filling synthesis and ligation. The DNA strand cleavage is catalyzed by a variety of AP-endonucleases (APE or HAP1, APEX, APE-1) that cleave 5' to the AP sites. APE also has an associated 3'-endonuclease activity the removes 3'trans-a,b-unsaturated aldehyde generated by AP lyase, as well as 3'-phosphate and 3'-phosphoglycolate. ROS generated 3'-blocked termini at the site of strand breaks are also removed APE.
Catalog #A2298-74C4
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlA431 lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to PKRGKKGAVAEDGD-C, from the N-terminus of the protein sequence according to NP_001632.2; NP_542379.1; NP_542380.1.
SpecificityRecognizes human APEX1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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