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Anti -Atg7, CT (Autophagy Protein 7, Ubiquitin-activating enzyme E1-like protein, hAGP7, APG7-like, ATG7, APG7L)
Anti -Atg7, CT (Autophagy Protein 7, Ubiquitin-activating enzyme E1-like protein, hAGP7,
APG7-like, ATG7, APG7L)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IC |
|
| Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1 (3). Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog (4). In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles (5). It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death (6). | | | Catalog # | A2298-74S | | Applications | Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5-1ug/ml | | Immunocytochemistry: 10ug/ml | | Optimal dilutions to be determined by the researcher. | | Positive Control | Caco-2 cell lysate | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide, 17aa from near the carboxy terminus of human APG7 (Genbank accession No. NP_006386). | | Specificity | Recognizes human APG7. Species Crossreactivity: mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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