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Anti -Atg4A (ATG4A, Autophagy Related Protein 4 Homolog A, Autophagin 2)
Anti -Atg4A (ATG4A, Autophagy Related Protein 4 Homolog A, Autophagin 2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
E B |
|
| ATG4A (Autophagy related protein 4 homolog A; also Autophagin 2) is a 50kD cytosolic cysteine protease mostly found in skeletal muscle and brain. It belongs to the Autophagin protein family which is involved in autophagy, a biological process in which endogenous protein and damaged organelles are destroyed. There is a potential for multiple splice forms, namely isoform 1, isoform 2, and isoform 3. Human ATG4A shares 92% and 94% aa sequence identity with mouse and canine ATG4A, respectively. | | | Catalog # | A2298-74V | | Applications | Suitable for use in Western Blot and ELISA. Other applications not tested. | | Recommended Dilution | Western Blot: 0.1-0.2ug/ml | | ELISA: 0.5-1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | As reported | | Form | Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol. | | Purity | Purified by human ATG4A affinity chromatography. | | Immunogen | Sf21-derived, recombinant human Autophagy related protein 4 homolog A isoform 1 (aa2-398) Accession # Q8WYN0 | | Specificity | Recognizes human ATG4A. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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