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You are here:Home » Antibodies » Antibodies-Autophagy » Anti -Atg4A (ATG4A, Autophagy Related Protein 4 Homolog A, Autophagin 2)

Anti -Atg4A (ATG4A, Autophagy Related Protein 4 Homolog A, Autophagin 2)


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Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B
ATG4A (Autophagy related protein 4 homolog A; also Autophagin 2) is a 50kD cytosolic cysteine protease mostly found in skeletal muscle and brain. It belongs to the Autophagin protein family which is involved in autophagy, a biological process in which endogenous protein and damaged organelles are destroyed. There is a potential for multiple splice forms, namely isoform 1, isoform 2, and isoform 3. Human ATG4A shares 92% and 94% aa sequence identity with mouse and canine ATG4A, respectively.
Catalog #A2298-74V
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-0.2ug/ml
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
PurityPurified by human ATG4A affinity chromatography.
ImmunogenSf21-derived, recombinant human Autophagy related protein 4 homolog A isoform 1 (aa2-398) Accession # Q8WYN0
SpecificityRecognizes human ATG4A.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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