Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Autophagy » Anti -ATG4D (ATG4 Autophagy Related 4 Homolog D, PG4D, APG4-D, AUTL4, AUT-like 4 Cysteine Endopeptidase, Autophagin-4, Autophagy-related Cysteine Endopeptidase 4, Autophagy-related Protein 4 Homolog D, Cysteine Protease ATG4D)

Anti -ATG4D (ATG4 Autophagy Related 4 Homolog D, PG4D, APG4-D, AUTL4, AUT-like 4 Cysteine
Endopeptidase, Autophagin-4, Autophagy-related Cysteine Endopeptidase 4, Autophagy-related
Protein 4 Homolog D, Cysteine Protease ATG4D)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.
Catalog #A2298-74V6
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA:1:32000
Western Blot:1ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHuman Skeletal Muscle lysates
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See A2298-74V6-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide, CSQLAQPTLRLPRT from the internal region (near C-terminus) of human ATG4D.
SpecificityRecognizes human ATG4D at ~26.2kD. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 52.9kD. Species sequence homology: mouse, rat, canine, bovine, porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links