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You are here:Home » Antibodies » Abs to Autophagy » Anti -Atg3 (Autophagy-related Protein 3, APG3, ATG3 Autophagy Related 3 Homolog (S. cerevisiae), APG3-like, AUT1, Protein PC3-96)

Anti -Atg3 (Autophagy-related Protein 3, APG3, ATG3 Autophagy Related 3 Homolog (S.
cerevisiae), APG3-like, AUT1, Protein PC3-96)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IH IF
GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. Belongs to the ATG3 family.
Catalog #A2298-74W
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 1:50-1:3000
Immunofluorescence: 1:50-1:3000
Western Blot: 1:50-1:3000
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a lyophilized powder. Reconstitute with 100ul of sterile ddH2O.
PuritySerum
ImmunogenSynthetic peptide corresponding to the central region of human ATG3, conjugated to Blue Carrier Protein. Cellular Localization
SpecificityRecognizes human ATG3 at ~37kD.
Species CrossreactivityRat and mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesAutophagy-related E2-like conjugation enzyme ATG3


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