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You are here:Home » Antibodies » Abs to Enzymes, Protease (Proteinase, Peptidase) » Anti -APEH, NT (Acylamino-acid-releasing Enzyme, AARE, Acyl-peptide Hydrolase, APH, Acylaminoacyl-peptidase, Oxidized Protein Hydrolase, OPH, DNF15S2, D3S48E, D3F15S2)

Anti -APEH, NT (Acylamino-acid-releasing Enzyme, AARE, Acyl-peptide Hydrolase, APH,
Acylaminoacyl-peptidase, Oxidized Protein Hydrolase, OPH, DNF15S2, D3S48E, D3F15S2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B
APEH is the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. APEH gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300kD with each subunit consisting of 732 amino acid residues. The protein can play an important role in destroying oxidatively damaged proteins in living cells.
Catalog #A2298-74X
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:1,000
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by ammonium sulfate precipitation.
ImmunogenSynthetic peptide selected from the N-terminal region of human APEH (KLH).
SpecificityRecognizes human APEH.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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