Anti -Apg12 (Autophagy-related 12, 4931423H11Rik, A330058M13Rik, Atg12, Apg12L,
APG12-like, Atg12L, Autophagy-related protein 12)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IP |
|
| Autophagy is the major pathway for proteins and organelles being delivered to the lysosome or vacuole for degradation. Autophagy-mediated degradation may be important during mammalian embryogenesis, differentiation, aging, and non-apoptotic programmed cell death. Dysfunction in autophagic degradation has been observed in a number of genetic diseases, particularly in cancer, and may also be associated with neurodegenerative conditions such as Parkinson’s and Alzheimer’s disease. The Apg12 system is a ubiquitin-like protein conjugation system, first discovered in autophagy-defective yeast mutants, that is conserved in eukaryotes. Covalent attachment of Apg12-Apg5 is required for autophagy; the Apg7 and Apg10 enzymes that catalyze this attachment are analogous to the E1 and E2 enzymes of the ubiquitin-proteasome degradation pathway. | | | Catalog # | A2298-75A | | Applications | Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested. | | Recommended Dilutions | ELISA: 0.1-1ug/ml | | Immunoprecipitation (Native): 7ug/reaction | | Western Blot: 1-3ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.25mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide derived from the internal region of the human Apg12 protein. | | Specificity | Recognizes human Apg12 protein at ~22kD, as well as additional bands between 40 and 80kD of unknown origin. Reactivity has been confirmed with Apg12-transfected, overexpressed HEK293 cell lysates. Species Sequence Homology: mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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